Preservation with RNAlater

Preservation for RNA/DNA (using RNAlater)

 

This commercially available fixative is often used with success; other labs find it problematic. It is apparently not a good means for preserving microRNAs. Best practices are as follows:

A) Maintain a fixative:tissue ratio of 5:1 at least. More fixative is better.

B) Cut the tissue into very small pieces, e.g., 1-2 mm cubes.

C) Incubate overnight at room temperature.

D) Store at -20°C or -80°C.

 

 

 

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